SrasV1, Main, Exploration, bibRecord, 002F15

RNA aptamer-based sensitive detection of SARS coronavirus nucleocapsid protein

Identifieur interne : 002F15 ( Main/Exploration ); précédent : 002F14; suivant : 002F16

RNA aptamer-based sensitive detection of SARS coronavirus nucleocapsid protein

Auteurs : Dae-Gyun Ahn [Corée du Sud] ; Il-Ji Jeon [Corée du Sud] ; JUNG DONG KIM [Corée du Sud] ; Min-Sun Song [Corée du Sud] ; Seung-Ryul Han [Corée du Sud] ; Seong-Wook Lee [Corée du Sud] ; Hyungil Jung [Corée du Sud] ; Jong-Won Oh [Corée du Sud]

Source :

Analyst : (London. 1877. Print) [ 0003-2654 ] ; 2009.

RBID : Pascal:09-0485565

Descripteurs français

Pascal (Inist)
- Marqueur, Enrichissement chimique, Constante dissociation, Mobilité électrophorétique, Analyse chimique, Chimiluminescence, Composé ultratrace, Méthode immunologique, Protéine, Anticorps.

English descriptors

KwdEn :
- Antibody, Chemical analysis, Chemical enrichment, Chemiluminescence, Dissociation constant, Electrophoretic mobility, Immunological method, Marker, Protein, Ultratrace compound.

Abstract

Severe acute respiratory syndrome coronavirus (SARS-CoV) is the etiological agent of a newly emerged disease SARS. The SARS-CoV nucleocapsid (N) protein is one of the most abundant structural proteins and serves as a diagnostic marker for accurate and sensitive detection of the virus. Using a SELEX (systematic evolution of ligand by exponential enrichment) procedure and recombinant N protein, we selected a high-affinity RNA aptamer capable of binding to N protein with a dissociation constant of 1.65 nM. Electrophoretic mobility shift assays and RNA competition experiments showed that the selected aptamer recognized selectively the C-terminal region of N protein with high specificity. Using a chemiluminescence immunosorbent assay and a nanoarray aptamer chip with the selected aptamer as an antigen-capturing agent, we could sensitively detect N protein at a concentration as low as 2 pg/ml. These aptamer-antibody hybrid immunoassays may be useful for rapid, sensitive detection of SARS-CoV N protein.

Affiliations:

Links toward previous steps (curation, corpus...)

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Le document en format XML

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<front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome coronavirus (SARS-CoV) is the etiological agent of a newly emerged disease SARS. The SARS-CoV nucleocapsid (N) protein is one of the most abundant structural proteins and serves as a diagnostic marker for accurate and sensitive detection of the virus. Using a SELEX (systematic evolution of ligand by exponential enrichment) procedure and recombinant N protein, we selected a high-affinity RNA aptamer capable of binding to N protein with a dissociation constant of 1.65 nM. Electrophoretic mobility shift assays and RNA competition experiments showed that the selected aptamer recognized selectively the C-terminal region of N protein with high specificity. Using a chemiluminescence immunosorbent assay and a nanoarray aptamer chip with the selected aptamer as an antigen-capturing agent, we could sensitively detect N protein at a concentration as low as 2 pg/ml. These aptamer-antibody hybrid immunoassays may be useful for rapid, sensitive detection of SARS-CoV N protein.</div>
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RNA aptamer-based sensitive detection of SARS coronavirus nucleocapsid protein

RNA aptamer-based sensitive detection of SARS coronavirus nucleocapsid protein

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri